Auramine O


Auramine 'O' is hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Zieh Neelsen. It also can be used to make a fluorescent Schiff reagent.


Auramine 'O' MSDS
Auramine O Manufacturer India

Auramine O Specifications

Chemical Name Auramine O
Chemical Formula C17H21N3
Molecular Weight 267.373
Moistuer 1.5% w/w max
Physical Appearance Yellow Powder
C.I. Name Basic Yellow 2
C.I. Number 41000
CAS Number 2465-27-2
Boiling Point(C) 136
pH(1 g / 99 mL water) 5.8-7.5
Ammonical Nitrogen as NH4 HSO4 0.15
Synonyms of Auramine O: SAuramine O, Basic yellow 2, Pyocatanium
aureum, aizen auramine, Pyoktanin Yellow,
Canary Yellow, Pyoktanin, C.I. 41000

RaviChem Industries as Auramine O manufacturer

Raviraj Chemicals is one of the well known Auramine O manufacturer in India. We are manufacturing and exporting Auramine O from more than one decade. Our manufactured Auramine O is known for its accurate chemical specifications, which makes it worthy to apply in varied industrial processes.

Applications Of Auramine O

  • Auramine O which is also called Basic yellow 2 is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals. It is very soluble in water and soluble in ethanol.
  • Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium), where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain. It can also be used as a fluorescent version of Schiff reagent.
  • Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis. It can be also used as an antiseptic agent.
  • Auramine O can be used in the dyeing of acrylic fibres, silk and cotton fiber; also applied in the dyeing of leather, paper, hemp and viscose; Auramine O can also be used in the direct printing and blotch discharge printing of tannin mordant dyed cotton, and also used to produce colour lake and used in inks.

Handling and Storage

Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Separate from incompatibilities. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.